Journal: Pathology

Article Title: Overexpression of HOMER2 predicts better outcome in low-grade endometrioid endometrial adenocarcinoma

doi: 10.1016/j.pathol.2018.03.004

Figure Lengend Snippet: (A) Strong (2–3+) immunohistochemical staining with HOMER2. (B) Negative (0–1+) immunohistochemical staining with HOMER2.

Article Snippet: Antigen retrieval was performed by heating in the microwave for 20 min, before incubating the sections with a polyclonal recombinant protein HOMER2 antibody (1:50, 60 min; Novus Biologicals, USA).

Techniques: Immunohistochemical staining, Staining

Journal: Pathology

Article Title: Overexpression of HOMER2 predicts better outcome in low-grade endometrioid endometrial adenocarcinoma

doi: 10.1016/j.pathol.2018.03.004

Figure Lengend Snippet: Correlation of HOMER2 expression and clinical and pathological data

Article Snippet: Antigen retrieval was performed by heating in the microwave for 20 min, before incubating the sections with a polyclonal recombinant protein HOMER2 antibody (1:50, 60 min; Novus Biologicals, USA).

Techniques: Expressing

Journal: Pathology

Article Title: Overexpression of HOMER2 predicts better outcome in low-grade endometrioid endometrial adenocarcinoma

doi: 10.1016/j.pathol.2018.03.004

Figure Lengend Snippet: Survival curve for group 1 cohort showing better cause-specific survival in patients with a strong HOMER2 expression.

Article Snippet: Antigen retrieval was performed by heating in the microwave for 20 min, before incubating the sections with a polyclonal recombinant protein HOMER2 antibody (1:50, 60 min; Novus Biologicals, USA).

Techniques: Expressing

Journal: Pathology

Article Title: Overexpression of HOMER2 predicts better outcome in low-grade endometrioid endometrial adenocarcinoma

doi: 10.1016/j.pathol.2018.03.004

Figure Lengend Snippet: Multivariate analysis for endometrial cancer-specific survival in endometrioid adenocarcinoma

Article Snippet: Antigen retrieval was performed by heating in the microwave for 20 min, before incubating the sections with a polyclonal recombinant protein HOMER2 antibody (1:50, 60 min; Novus Biologicals, USA).

Techniques:

Journal: Scientific Reports

Article Title: Live Neuron High-Content Screening Reveals Synaptotoxic Activity in Alzheimer Mouse Model Homogenates

doi: 10.1038/s41598-020-60118-y

Figure Lengend Snippet: Validation of PSD95-mVenus postsynaptic, VAMP2-mRFP presynaptic, and colocalized puncta. The majority of anti-VAMP2 and anti-Synapsin1 stained puncta were colocalized with endogenous VAMP2-mRFP puncta. Similarly, the majority of the antibody stained anti-PSD95 and anti-Homer2 puncta were colocalized with endogenous PSD95-mVenus puncta. ( a–c ) Presynaptic VAMP2-mRPF in singly transgenic mice was validated with immunocytochemistry stained ( a ) anti-VAMP2 and ( b ) anti-Syn1, ( c ) 92.1 ± 4.8% of stained VAMP2 puncta and 80.8 ± 3.9% of stained Syn1 puncta was colocalized with endogenous VAMP2-mRFP; ( d–f ) Postsynaptic PSD95-mVenus expression in singly transgenic mice was validated with immunocytochemistry stained ( d ) anti-PSD95 and ( e ) anti-Homer2, ( f ) 107.5 ± 4.5% of stained PSD95 puncta and 113.7 ± 6.2% of stained Homer2 puncta was colocalized with endogenous PSD95-mVenus (mean ± SD%, n = 3).

Article Snippet: All cells were blocked with 5% bovine serum albumin and 0.2% Triton X-100 (Sigma-Aldrich) in PBS for 1 hour at room temperature, and then incubated with anti-Synaptobrevin 2, anti-Synapsin1, anti-PSD95, or anti-Homer2 (Synaptic Systems) antibodies at 1:500 in blocking solution overnight at 4 °C, subsequently washed in 0.1% Triton X-100 in PBS three times.

Techniques: Staining, Transgenic Assay, Immunocytochemistry, Expressing

Journal: Nature neuroscience

Article Title: Synaptic depression via mGluR1 positive allosteric modulation suppresses cue-induced cocaine craving

doi: 10.1038/nn.3590

Figure Lengend Snippet: (a) Co-immunoprecipitation (co-IP) experiments assessing the physical associations between mGluR1 and Homer proteins on withdrawal day (WD) 14, 25 or 48 from extended-access cocaine (coc) or saline (sal) self-administration (see timeline in ). No significant changes in association between mGluR1 and Homer proteins were observed at any of the 3 withdrawal time-points. ( b ) In the case of mGluR5, no change in association with Homer proteins was observed at the two earlier withdrawal time-points (WD14 and WD25). However, a significant decrease in association between mGluR5 and both Homer isoforms was found on WD48 in animals that previously self-administered cocaine. Data are expressed as percent of Saline group (± s.e.m.) at each time-point (n values are provided within each bar). **p=0.01 (Homer1bc) and *p=0.04 (Homer2) versus respective Saline groups. Full-length blots are presented in .

Article Snippet: Validation is provided on the supplier’s website for all antibodies utilized, as well as on Antibodypedia for Homer1bc (Santa Cruz) and Homer2 (Abnova).

Techniques: Immunoprecipitation, Co-Immunoprecipitation Assay, Saline

Journal: Nature neuroscience

Article Title: Synaptic depression via mGluR1 positive allosteric modulation suppresses cue-induced cocaine craving

doi: 10.1038/nn.3590

Figure Lengend Snippet: ( a ) Timeline (SA, self-administration). To assess the effects of Homer overexpression on incubation of cocaine craving, viruses (AAV-GFP, Homer1c or Homer2) were injected into the NAc core at the same time that animals underwent jugular catheterization surgery, allowing Homer expression to peak during early withdrawal. ( b ) Staining for the HA tag on cDNA-Homer1c verified localized overexpression in the NAc core (20X magnification; image taken ~3 months after virus injection; scale bar, 100μm; ac, anterior commissure). ( c ) Following ~1 week of recovery from surgeries, animals self-administered cocaine (0.5 mg/kg) for 6 h/day for 10 days. No difference in cocaine intake (average infusions obtained each day ± s.e.m.) was observed between the 3 groups. ( d ) All animals showed an increase in cue-induced cocaine-seeking on WD48 compared to WD1 (i.e., incubation), regardless of which virus infusion they received. ***p<0.001, **p=0.01, WD1 versus WD48 (ANOVA followed by least significant difference post-hoc comparisons); GFP, n=10 rats; Homer1c, n=8 rats; Homer2, n=10 rats.

Article Snippet: Validation is provided on the supplier’s website for all antibodies utilized, as well as on Antibodypedia for Homer1bc (Santa Cruz) and Homer2 (Abnova).

Techniques: Over Expression, Incubation, Injection, Expressing, Staining, Virus